Increased β-glucuronidase activity in bronchoalveolar lavage fluid of children with bacterial lung infection: A case-control study
| Autor: | Evgenia C. Panagiotopoulou, Michael B. Anthracopoulos, Kostas N. Priftis, Irene-Eva Triantaphyllidou, Konstantinos Douros, Sotirios Fouzas, Christina J. Malavaki, Nikos K. Karamanos |
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| Rok vydání: | 2015 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty education.field_of_study Necrosis medicine.diagnostic_test business.industry Peritoneal fluid Elastase Population Area under the curve Gastroenterology Bronchoalveolar lavage Interquartile range Internal medicine Immunology Extracellular fluid Medicine medicine.symptom business education |
| Zdroj: | Respirology. 20:1248-1254 |
| ISSN: | 1323-7799 |
| DOI: | 10.1111/resp.12596 |
| Popis: | Background and objective β-Glucuronidase is a lysosomal enzyme released into the extracellular fluid during inflammation. Increased β-glucuronidase activity in the cerebrospinal and peritoneal fluid has been shown to be a useful marker of bacterial inflammation. We explored the role of β-glucuronidase in the detection of bacterial infection in bronchoalveolar lavage fluid (BALF) of paediatric patients. Methods In this case–control study, % polymorphonuclear cell count (PMN%), β-glucuronidase activity, interleukin-8 (IL-8), tumour necrosis factor-α (TNF-α) and elastase were measured in culture-positive (≥104 cfu/mL, C+) and -negative (C−) BALF samples obtained from children. Results A total of 92 BALF samples were analysed. The median β-glucuronidase activity (measured in nanomoles of 4-methylumbelliferone (4-MU)/mL BALF/h) was 246.4 in C+ (interquartile range: 71.2–751) and 21.9 in C− (4.0–40.8) (P |
| Databáze: | OpenAIRE |
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