Identification and development of eight microsatellite markers for the gypsophyte Helianthemum squamatum and cross-species amplification

Autor:	Adrián Escudero, Bernardo de Haro Reyes, Alfredo García-Fernández
Rok vydání:	2014
Předmět:	Ecology Biodiversity Population genetics Cistaceae Biology biology.organism_classification Helianthemum cinereum humanities Loss of heterozygosity Botany Genetics Helianthemum squamatum Microsatellite Identification (biology) Ecology Evolution Behavior and Systematics
Zdroj:	Conservation Genetics Resources. 6:1049-1051
ISSN:	1877-7260 1877-7252
DOI:	10.1007/s12686-014-0285-6
Popis:	Helianthemum squamatum (L.) Dum. Cours. (Cistaceae) is an endemic specialist plant that inhabits the gypsum soils of the semi-arid Iberian Peninsula, one of the ecosystems most vulnerable to climate change. In this study, eight microsatellite markers were isolated and developed using next-generation sequencing data in order to provide a validated tool for genetic studies of this species. Their polymorphisms were tested in two populations. The number of alleles and the expected heterozygosity ranged from 2 to 8 and from 0.29 to 0.82 respectively. Cross-amplification was carried out in the related congeners Helianthemum hirtum and Helianthemum cinereum.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bfe1869beb5a2b3d04a7bf3b716e0bf8 https://doi.org/10.1007/s12686-014-0285-6 Zobrazit plný text záznamu Full text from SpringerLink