Characterization and anti-tumor activity of a polysaccharide from Hedysarum polybotrys Hand.-Mazz
| Autor: | Xue-xi Wang, Shi-gang Li, Zhong Liu, Wei Tian, De-gui Wang, Jian-xiong Zhao, Ru Chen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Chromatography Polymers and Plastics Organic Chemistry Periodate Biological activity Polysaccharide High-performance liquid chromatography In vitro Hot water extraction Residue (chemistry) chemistry.chemical_compound chemistry Biochemistry Materials Chemistry Glucan |
| Zdroj: | Carbohydrate Polymers. 73:344-350 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2007.12.001 |
| Popis: | A water-soluble polysaccharide named as HPS-1 was isolated from the roots of Hedysarum polybotrys Hand.-Mazz by hot water extraction, anion-exchange and gel-permeation chromatography and tested for its anti-tumor activity. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, GLC–MS, methylation analysis, periodate oxidation and Smith degradation. Based on the data obtained, HPS-1 was found to be an α-(1 → 4)- d -glucan, with a single α- d -glucose at the C-6 position every nine residue, on average, along the main chain. The glucan has a weight-average molecular weight of about 9.4 × 10 4 Da. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay revealed that HPS-1 significantly inhibited the proliferation of human hepatocellular carcinoma HEP-G2 cells and human gastric cancer MGC-803 cells in vitro, indicating HPS-1 could have a possible cancer therapeutic potential. |
| Databáze: | OpenAIRE |
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