HPLC-MS Method for Simultaneous Quantitative Determination of an Innovative Russian Gestagen and its Metabolites in Rat and Rabbit Blood Sera

Autor:	V. A. Votyakov, E. S. Stepanova, V. A. Parshin, N. L. Shimanovskii, Yu. L. Rybakov, T. A. Fedotcheva, V. M. Gukasov, L. M. Makarenkova, V. V. Chistyakov
Rok vydání:	2019
Předmět:	Pharmacology Detection limit Analyte Chromatography 010405 organic chemistry Chemistry Calibration curve Extraction (chemistry) Rabbit (nuclear engineering) Standard solution 01 natural sciences High-performance liquid chromatography Quantitative determination 0104 chemical sciences 010404 medicinal & biomolecular chemistry Drug Discovery
Zdroj:	Pharmaceutical Chemistry Journal. 52:1016-1020
ISSN:	1573-9031 0091-150X
DOI:	10.1007/s11094-019-01944-x
Popis:	An HPLC-MS method for simultaneous quantitative determination of a novel gestagenic pharmaceutical and two of its metabolites in rat and rabbit blood sera was developed and validated. The method was selective and specific. The detection limit for all analytes was 5 ng/mL; lower limit of quantitation, 10 ng/mL. Calibration curves for all analytes had the form y = ax + b. The correlation coefficients were >0.99. Matrix effects, degree of extraction, and stability of analytes in standard solutions, analytical samples, and the biomatrix with multiple freeze—thaw cycles were studied.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bfcb9a5ec8ea654c716c8459f12b6d17 https://doi.org/10.1007/s11094-019-01944-x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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