| Autor: |
Martin F. Lavin, G. Coughlin, Robert A. Gardiner, Christoph Zenzmaier, Horst Joachim Schirra, James W.F. Catto, L. Teng, T. Gianduzzo, Peter Berger, Marion Buck, Hemamali Samaratunga, John F. Hancock, Raymond A. Clarke, John Yaxley |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
BJU International. 107:6-31 |
| ISSN: |
1464-4096 |
| Popis: |
In our commitment to developing a non-invasive test for prostate cancer detection, we identifi ed a short list of discriminating mRNA markers in prostatic tissues to complement the non-coding gene PCA3 for use in molecular profiling of prostatic fluid. RT-PCR for PCA3 and Hepsin from ejaculate together with serum PSA provided a sensitivity of 75% with a specifi city of 85% in the detection of prostate cancer from 75 patients. Since the addition of further mRNA markers only marginally improved these results, we have extended our investigations to include microRNA, metabonomic and Dkk-3 protein analyses. BMMC1, the gene in which PCA3 is embedded, is also being examined to establish binding partners to determine the role of this gene in prostate cancer. Preliminary analyses of multiple parameters with metabonomics of ejaculate from 80 patients have demonstrated Gleason score to be most closely aligned as clinical parameter. However, serum PSA by itself was positioned at almost 90 degrees off from the axis delineating cancer status and cancer severity indicating its lack of relationship with severity of cancer. Dkk-3, one of the secreted glycoproteins of the Dickkopf family, also promises to be a useful discriminator with Dkk-3 levels in 80 patients’ ejaculates being signifi cantly different in prostate cancer and TRUS negative patient specimens. An update of our integrated marker findings from ejaculate specimens obtained from ≥100 patients between 2007 and 2010 will be presented. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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