Validation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test
| Autor: | Franklin Riet-Correa, Pablo Parodi, Rodolfo Rivero, Luis Gustavo Corbellini, María T. Armúa-Fernández, José M. Venzal, Cecilia Miraballes, Vanessa Bielefeldt Leotti |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Tick-borne disease General Veterinary animal diseases 030231 tropical medicine Babesia bovis Babesiosis Biology medicine.disease biology.organism_classification Virology 030308 mycology & parasitology 03 medical and health sciences 0302 clinical medicine Anaplasma marginale parasitic diseases Multiplex polymerase chain reaction Babesia medicine Anaplasmosis |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 33:73-79 |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent. |
| Databáze: | OpenAIRE |
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