1H NMR metabolic fingerprinting of saffron extracts

Autor:	Nils T. Nyberg, Javad Asili, Ali Yilmaz, Per Mølgaard, Jerzy W. Jaroszewski
Rok vydání:	2010
Předmět:	chemistry.chemical_compound Chromatography chemistry Endocrinology Diabetes and Metabolism Clinical Biochemistry Crocetin Principal component analysis Proton NMR Biochemistry Picrocrocin Statistical correlation
Zdroj:	Metabolomics. 6:511-517
ISSN:	1573-3890 1573-3882
Popis:	The aim of this study was to explore feasibility of 1H NMR metabolic fingerprinting for discrimination of authenticity of saffron using principal component analysis (PCA) modeling. Authentic reference Iranian saffron (n = 31) and commercial samples (n = 32) were used. Cross-validated PCA models based on 1H NMR spectra of solutions prepared by direct extraction of grinded saffron with methanol-d4 distinguished reference Iranian saffron samples from commercial samples that formed several distinct clusters, some of which represent falsified samples as confirmed by microscopic analysis. The production sites and drying conditions of the authentic reference Iranian samples were not reflected in the current dataset. Picrocrocin and glycosyl esters of crocetin emerged as the most important 1H NMR markers of authentic saffron by using statistical correlation spectroscopy. In conclusion, 1H NMR spectra of saffron extracts combined with pattern recognition by PCA provide immediate means of unsupervised classification of saffron samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bfb690a909262b18daea04d4d8741098 https://doi.org/10.1007/s11306-010-0221-z Zobrazit plný text záznamu Plný text ve formátu PDF