Temporal changes of symbiont density and host fitness after rifampicin treatment in a whitefly of theBemisia tabacispecies complex
| Autor: | Chang-Rong Zhang, Hai-Qin Tang, Shu-Sheng Liu, Ting-Ting Yan, Hong-Wei Shan, Yin-Quan Liu, Xiao-Wei Wang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
animal structures medicine.drug_class Population Antibiotics Whitefly General Biochemistry Genetics and Molecular Biology Microbiology 03 medical and health sciences Botany medicine Mating education Ecology Evolution Behavior and Systematics education.field_of_study biology Host (biology) fungi food and beverages biochemical phenomena metabolism and nutrition biology.organism_classification 030104 developmental biology Rickettsia Insect Science bacteria Instar PEST analysis Agronomy and Crop Science |
| Zdroj: | Insect Science. 23:200-214 |
| ISSN: | 1672-9609 |
| DOI: | 10.1111/1744-7917.12276 |
| Popis: | Microbial symbionts are essential or important partners to phloem-feeding insects. Antibiotics have been used to selectively eliminate symbionts from their host insects and establish host lines with or without certain symbionts for investigating functions of the symbionts. In this study, using the antibiotic rifampicin we attempted to selectively eliminate certain symbionts from a population of the Middle East-Asia Minor 1 whitefly of the Bemisia tabaci species complex, which harbors the primary symbiont "Candidatus Portiera aleyrodidarum" and two secondary symbionts "Candidatus Hamiltonella defensa" and Rickettsia. Neither the primary nor the secondary symbionts were completely depleted in the adults (F0) that fed for 48 h on a diet treated with rifampicin at concentrations of 1-100 μg/mL. However, both the primary and secondary symbionts were nearly completely depleted in the offspring (F1) of the rifampicin-treated adults. Although the F1 adults produced some eggs (F2), most of the eggs failed to hatch and none of them reached the second instar, and consequently the rifampicin-treated whitefly colony vanished at the F2 generation. Interestingly, quantitative polymerase chain reaction assays showed that in the rifampicin-treated whiteflies, the density of the primary symbiont was reduced at an obviously slower pace than the secondary symbionts. Mating experiments between rifampicin-treated and untreated adults demonstrated that the negative effects of rifampicin on host fitness were expressed when the females were treated by the antibiotic, and whether males were treated or not by the antibiotic had little contribution to the negative effects. These observations indicate that with this whitefly population it is not feasible to selectively eliminate the secondary symbionts using rifampicin without affecting the primary symbiont and establish host lines for experimental studies. However, the extinction of the whitefly colony at the second generation after rifampicin treatment indicates the potential of the antibiotic as a control agent of the whitefly pest. |
| Databáze: | OpenAIRE |
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