Enzymatic esterification in ionic liquids integrated with pervaporation for water removal

Autor:	László Gubicza, Nándor Nemestóthy, Tamás Fráter, Katalin Bélafi-Bakó
Rok vydání:	2003
Předmět:	biology Water activity Ionic bonding Alcohol Pollution Candida rugosa chemistry.chemical_compound chemistry Ionic liquid biology.protein Environmental Chemistry Organic chemistry Thermal stability Pervaporation Lipase
Zdroj:	Green Chemistry. 5:236
ISSN:	1463-9270 1463-9262
DOI:	10.1039/b211342m
Popis:	The application of ionic liquids as solvents for the enantioselective esterification of (R,S)-2-chloropropanoic acid with butan-1-ol using Candida rugosa lipase is reported. The role of water produced during the reaction and controlling of the water activity with pervaporation was studied. The enantioselective esterification of (R,S)-2-chloropropanoic acid with butan-1-ol in different organic solvents and ionic liquids was studied. The reaction was effectively catalysed by Candida rugosa lipase. From the ionic solvents investigated, the best results were achieved in [bmim]PF6. The presence of water had a strong effect on the activity of the lipase. Since in esterification reactions with acid and alcohol equimolar amounts of water are formed, the excess of water was removed and the water activity was kept constant using a pervaporation system without any additives. At the optimum water activity Candida rugosa lipase showed high thermal stability in [bmim]PF6 and it could be reused for at least five recycles with only a small lost of activity.
Databáze:	OpenAIRE
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