Phosphorus-31 nuclear magnetic resonance studies on serum low and high density lipoproteins. Effect of paramagnetic ion

Autor:	Thomas Glonek, Angelo M. Scanu, Arthur W. Kruski, Leonard G. Davis, Thomas O. Henderson
Rok vydání:	1975
Předmět:	Vesicle Analytical chemistry Phospholipid Phosphate Biochemistry chemistry.chemical_compound Blood serum Nuclear magnetic resonance chemistry Reagent Phosphatidylcholine lipids (amino acids peptides and proteins) Phosphorus-31 NMR spectroscopy Titration
Zdroj:	Biochemistry. 14:1915-1920
ISSN:	1520-4995 0006-2960
Popis:	A paramagnetic quenching reagent, Mn/sup 2 +//EDTA (1:22,2), was developed for the purpose of investigating the phospholipid phosphate groupings of human serum low and high density lipoproteins through the quenching effect of the reagent on the /sup 31/P nuclear magnetic resonance signals from these complexes. Systems investigated included native low and high density serum lipoproteins (LDL, HDL/sub 2/, and HDL/sub 3/), egg phosphatidylcholine vesicles together with appropriate phosphodiester model systems, diethyl phosphate in aqueous buffer, and phosphatidylcholine and sphingomyelin both in anhydrous methanol. The results of these studies indicated that ca. 50% of the phospholipid-phosphorus signal of LDL is quenched upon titration as compared to an 80-85% figure observed for HDL/sub 2/ and HDL/sub 3/. In all cases the spectral effects were totally reversible upon removal of the paramagnetic ion by dialysis. The results of the titration studies indicated a similar but not an identical behavior between HDL/sub 2/ and HDL/sub 3/. The results are consistent with model structures of HDL and LDL particles derived from low angle X-ray diffraction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bfa87f3df5cd061915be3eb5efaefb0a https://doi.org/10.1021/bi00680a017 Zobrazit plný text záznamu