Exploring the evolution and epidemiology of European CC1-MRSA-IV: tracking a multidrug-resistant community-associated meticillin-resistant Staphylococcus aureus clone
| Autor: | T Vremera, Eike J. Steinig, CP Aymerich, David C. Coleman, Earls, Sebastián Duchêne, Stefan Monecke, A Lacoma, Olivia Dorneanu, M. Armengol-Porta, Jas Castruita, Bartels, Alexandra Simbeck, Anita Blomfeldt, Wulf Schneider-Brachert, Ralf Ehricht, Igor Loncaric, M Bes, U Wernery |
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| Rok vydání: | 2021 |
| Předmět: |
Genetics
0303 health sciences education.field_of_study Lineage (genetic) Phylogenetic tree 030306 microbiology SCCmec Population Clone (cell biology) General Medicine Biology medicine.disease_cause 03 medical and health sciences Staphylococcus aureus Phylogenetics medicine education Genotyping 030304 developmental biology |
| Zdroj: | Microbial Genomics. 7 |
| ISSN: | 2057-5858 |
| DOI: | 10.1099/mgen.0.000601 |
| Popis: | This study investigated the evolution and epidemiology of the community-associated and multidrug-resistant Staphylococcus aureus clone European CC1-MRSA-IV. Whole-genome sequences were obtained for 194 European CC1-MRSA-IV isolates (189 of human and 5 of animal origin) from 12 countries, and 10 meticillin-susceptible precursors (from North-Eastern Romania; all of human origin) of the clone. Phylogenetic analysis was performed using a maximum-likelihood approach, a time-measured phylogeny was reconstructed using Bayesian analysis, and in silico microarray genotyping was performed to identify resistance, virulence-associated and SCCmec (staphylococcal cassette chromosome mec) genes. Isolates were typically sequence type 1 (190/204) and spa type t127 (183/204). Bayesian analysis indicated that European CC1-MRSA-IV emerged in approximately 1995 before undergoing rapid expansion in the late 1990s and 2000s, while spreading throughout Europe and into the Middle East. Phylogenetic analysis revealed an unstructured meticillin-resistant S. aureus (MRSA) population, lacking significant geographical or temporal clusters. The MRSA were genotypically multidrug-resistant, consistently encoded seh, and intermittently (34/194) encoded an undisrupted hlb gene with concomitant absence of the lysogenic phage-encoded genes sak and scn. All MRSA also harboured a characteristic ~5350 nt insertion in SCCmec adjacent to orfX. Detailed demographic data from Denmark showed that there, the clone is typically (25/35) found in the community, and often (10/35) among individuals with links to South-Eastern Europe. This study elucidated the evolution and epidemiology of European CC1-MRSA-IV, which emerged from a meticillin-susceptible lineage prevalent in North-Eastern Romania before disseminating rapidly throughout Europe. |
| Databáze: | OpenAIRE |
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