Regulation of adenylyl cyclase signaling system by insulin, biogenic amines and glucagon at their separate and combined action in muscle membranes of mollusc Anodonta cygnea
| Autor: | Svetlana A. Plesneva, A. O. Shpakov, L. A. Kuznetsova, M. N. Pertseva, T. S. Sharova |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Physiology Insulin medicine.medical_treatment Stimulation Biology Pertussis toxin Biochemistry Glucagon Adenylyl cyclase chemistry.chemical_compound Endocrinology chemistry Internal medicine medicine Serotonin Ecology Evolution Behavior and Systematics 5-HT receptor Hormone |
| Zdroj: | Journal of Evolutionary Biochemistry and Physiology. 49:145-152 |
| ISSN: | 1608-3202 0022-0930 |
| Popis: | In the smooth muscles of mollusc Anodonta cygnea the regulatory action of hormones on adenylyl cyclase signaling system (ACSS) are realized through the receptors of serpentine type (biogenic amines, isoproterenol, glucagon) and receptor tyrosine kinase (insulin) type. Intracellular mechanisms of their interaction are interconnected. Application of hormones, their antagonists and pertussis toxin in combination with insulin and biogenic amines or glucagon on adenylyl cyclase (AC) activity allows revealing the possible sites of cross-linking in the mechanisms of their action. Combined influence of insulin and serotonin or glucagon leads to decreased stimulation of adenylyl cyclase (AC) by these hormones, whereas combined application of insulin and isoproterenol suppresses AC-stimulating effect of insulin, but AC-inhibiting effect of isoproterenol is maintained in the presence and absence of non-hydrolysable analog of GTP—guanylyl imido diphosphate (GIDP). The specific blockage of AC-stimulating effect of serotonin by cyproheptadine—antagonist of serotonin receptors, did not change AC stimulation by insulin. Beta-adrenoblockers (propranolol and alprenolol) prevent inhibition of AC activity by isoproterenol, but did not change AC stimulation by insulin. Pertussis toxin blocked AC-inhibiting effect of isoproterenol and weakened AC-stimulating action of insulin. Thus, in the muscles of Anodonta cygnea negative interaction between ACS have been revealed, which are realized under combined influence of insulin and serotonin or glucagon, most probably, at the level of receptor of serpentine type (serotonin, glucagon), whereas under action of insulin and isoproterenol at the level of Gi protein and AC interaction. |
| Databáze: | OpenAIRE |
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