Simultaneous Determination of Testosterone and its Major Metabolite Epitestosterone in Biological Fluids by HPLC
| Autor: | Victoria F. Samanidou, Eftichia G. Karageorgou, Ioannis N. Papadoyannis |
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| Rok vydání: | 2007 |
| Předmět: |
Detection limit
Chromatography Chemistry Metabolite Clinical Biochemistry Epitestosterone Analytical chemistry Pharmaceutical Science Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound medicine Sample preparation Solid phase extraction Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 30:1317-1331 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1080/10826070701274916 |
| Popis: | A reversed‐phase high performance liquid chromatographic (HPLC) method is developed and validated for the simultaneous determination of anabolic steroids: testosterone and its major metabolite, epitestosterone. The analytical column, Inertsil ODS‐2, 5 µm, 250×4 mm, was operated at ambient temperature. Isocratic elution was performed using 35% of A=buffer solution 0.11% CH3COOH‐CH3COONa 7.5 mmol/L, pH=4 and 65% of B=CH3CN, at a flow rate of 0.8 mL/min. Inlet pressure was 155 bar. UV detection was performed at 236 nm. The limit of detection was 0.02 ng per 20 µL injection volume, while linearity held up to 2 ng/µL. p‐Cresol was used as internal standard at a concentration of 2 ng/µL. Validation of the method was performed in terms of accuracy and precision: intra‐day assay (n=6) and inter‐day assay (n=3×5) and was found to be satisfactory, with high accuracy and precision results. The method was successfully applied to biological fluids after solid phase extraction (SPE) on Nexus cartridges. Recove... |
| Databáze: | OpenAIRE |
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