Synthesis and Characterization of a Micelle-Based pH Nanosensor with an Unprecedented Broad Measurement Range
| Autor: | Kristoffer Almdal, Lise N. Feldborg, Thomas Lars Andresen, E. K. Pramod Kumar |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Chemistry of Materials. 25:1496-1501 |
| ISSN: | 1520-5002 0897-4756 |
| DOI: | 10.1021/cm302922d |
| Popis: | A new cross-linked micelle pH nanosensor design was investigated. The nanosensor synthesis was based on self-assembly of an amphiphilic triblock copolymer, poly(ethylene glycol)-b-poly(2-amino ethyl methacrylate)-b-poly(coumarin methacrylate) (PEG-b-PAEMA-b-PCMA), which was synthesized by isolated macroinitiator atom transfer radical polymerization. Micelles were formed by PEG-b-PAEMA-b-PCMA self-assembly in water, giving micelles with an average diameter of 45 nm. The PCMA core was employed to utilize coumarin-based photoinduced cross-linking in the core of the micelles, which was performed by UV irradiation (320 nm < λ < 500 nm), and the progress of the cross-linking was monitored by UV spectroscopy. The formed cross-linked core–shell–corona micelle was converted into ratiometric pH nanosensors by binding the pH-sensitive fluorophores oregon green 488 and 2′,7′-bis-(2-carboxyethyl)-5-(and-6) carboxyfluorescein and a reference fluorophore Alexa 633 to the PAEMA shell region of the micelles. Fluorescence ... |
| Databáze: | OpenAIRE |
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