Effect of Hyperglycemia on Erythrocyte Carbonic Anhydrase and Lactic Acid in Type II Diabetic Subjects
| Autor: | Umar Alhaji Ismaila, Ameh Danladi Amodu, Mohammed Fakhruddeen, Atawodi Sunday Ene-Ojo, Salihu Ismail Ibrahim |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification medicine.medical_specialty biology Triglyceride Lactic acid 03 medical and health sciences chemistry.chemical_compound Red blood cell 030104 developmental biology Enzyme Endocrinology medicine.anatomical_structure chemistry Internal medicine Carbonic anhydrase medicine biology.protein Glycated hemoglobin Hemoglobin medicine.symptom Acidosis |
| Zdroj: | Journal of Diabetes Mellitus. :158-165 |
| ISSN: | 2160-5858 2160-5831 |
| Popis: | Background: Carbonic anhydrase (CA) is a ubiquitous enzyme catalyzing the reversible hydration of CO2 to and H+. CA plays a crucial role in CO2 transport, acid-base balance, and in linking local acidosis to O2 unloading from hemoglobin and also facilitates lactate shuttling across the monocarboxylate transporters (MCT). The study aimed to investigate the influence of hyperglycemia on erythrocyte carbonic anhydrase activity and lactic acid in type II diabetic patients. Method: Red blood cell carbonic anhydrase activity was determined in washed lysed-hemolysate by the action of the enzyme on the substrate p-nitrophenyl acetate. The absorbance of released p-nitrophenol was recorded at 345 nm. Glycated Hemoglobin was determined by ion exchange method (Spectrum Diagnostic Kit). Blood glucose, lactate, cholesterol and triglyceride were determined using Accutrend GCT meter (Roche, Germany) with cobias® test strips. Results: The present study showed that hyperglycemia significantly (p < 0.05) increased both erythrocyte carbonic anhydrase activity and blood lactate level in type II diabetic patients. Conclusion: We may conclude that hyperglycemia may be responsible for the increased activity of carbonic anhydrase activity and blood lactate concentration. |
| Databáze: | OpenAIRE |
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