Chlorogenic Acid inhibits proliferation, migration and invasion of pancreatic cancer cells via AKT/GSK-3β/β-catenin signaling pathway
| Autor: | Xiukun Lin, Lili Yin, Xiaoliang Chen, Binyu Liu, Jiale Tong, Jianing Bo, Miao Feng |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Recent Patents on Anti-Cancer Drug Discovery. 18 |
| ISSN: | 1574-8928 1077-2340 |
| DOI: | 10.2174/1574892818666230327134746 |
| Popis: | Background: Chlorogenic acid (CA, United States Patent No. 10772340), a natural biologically active food ingredient, displays potent antitumor activity against a variety of cancer cells. However, the mechanism underlying its anticancer effect is not well elucidated. Objective: In the present study, we hope to dissect the mechanism underlying the anticancer effects of CA in pancreatic cancer cells. Methods: The cytotoxicity of CA in pancreatic cancer cells was determined by MTT assay. Flow cytometry was performed to evaluate the cells apoptosis, while a clonogenic assay was carried out to check the colony formation of cancer cells. Transwell assay was performed to assess the cells migration and invasion. The protein expression of AKT/GSK-3β/β-catenin signaling pathway was detected by Western Blotting. Results: Our data indicated that CA inhibited the proliferation of PANC-28 and PANC-1 cells in a dose- and time-dependent manner. CA was able to inhibit colony formation, migration, and invasion ability and trigger apoptosis in PANC-28 and PANC-1 cells. Further study showed that CA down-regulated the expression of AKT, p-AKT(Thr308), p-GSK-3β(Ser9), β-catenin, N-cadherin, and vimentin while enhancing the expression of cleaved caspase 7 in PANC-28 and PANC-1 cells. Conclusion: Our study provides significant evidence that CA is able to inhibit the growth of pancreatic cancer via the AKT/GSK-3β/β-catenin signaling pathway. |
| Databáze: | OpenAIRE |
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