Studies on the Discrimination against Strontium by Bone Grown in Vitro

Autor:	Lengemann Fw
Rok vydání:	1960
Předmět:	Bone mineral Calcium metabolism Alkaline earth metal Strontium Radiochemistry chemistry.chemical_element Cell Biology Metabolism Biology Calcium Biochemistry In vitro Blood serum chemistry Molecular Biology
Zdroj:	Journal of Biological Chemistry. 235:1859-1862
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(18)69324-x
Popis:	Culturing of embryonic bone in vitro showed that both chick and rat bone could discriminate against strontium in favor of calcium. The ends of the bones showed a lower Sr/sup 89//Ca/sup 45/ ratio than the shafts. The degree of selection was not dependent upon the species of origin of the serum used in the medium or upon the age of the bones. Heat-treating of the bones destroyed the ability to select against strontium; for live bone the mechanism functioned at 37 deg but not at 4 deg and 25 deg . Selection occurred at some point after the initial entry of the alkaline earth metals into the bone and was associated with the incorporation of the radioisotopes into a less labile form of bone mineral. Loss of radioactivity from this less labile fraction occurred only with viable bone at 37 deg . The Sr/sup 89/Ca/sup 45/ ratio of the bones relative to the same ratio for the medium as well as the total uptake of radioisotopes could be varied by incorporating enzyme inhibitors into the medium. (auth)
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bf7069d596b4f4c0af124b0e8166de58 https://doi.org/10.1016/s0021-9258(18)69324-x Zobrazit plný text záznamu