Hypothalamic Astrocytes Respond to Transforming Growth Factor-α with the Secretion of Neuroactive Substances That Stimulate the Release of Luteinizing Hormone-Releasing Hormone1
| Autor: | Florence Rage, Ying J. Ma, Karin Berg-von der Emde, William C. Wetsel, Sergio R. Ojeda |
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| Rok vydání: | 1997 |
| Předmět: |
endocrine system
medicine.medical_specialty Gonadotropin-releasing hormone Biology Peptide hormone Chemically defined medium Endocrinology medicine.anatomical_structure Hypothalamus Epidermal growth factor Internal medicine medicine Receptor hormones hormone substitutes and hormone antagonists Transforming growth factor Astrocyte |
| Zdroj: | Endocrinology. 138:19-25 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.138.1.4863 |
| Popis: | Previous studies demonstrated the involvement of transforming growth factor-α (TGFα), a member of the epidermal growth factor (EGF) family, in the developmental regulation of hypothalamic LHRH release. Although both TGFα and EGF stimulate LHRH release, they do not appear to act directly on LHRH neurons, as no EGF/TGFα receptors are detected on these cells in vivo. Instead, the stimulatory effect of TGFα on LHRH release seems to require a glial intermediacy. The present study identifies one of the glial molecules involved in this process. In vitro exposure of purified hypothalamic astrocytes to TGFα or EGF in a defined medium led to activation of the cyclooxygenase-mediated pathway of arachidonic acid metabolism, as indicated by an increase in PGE2 release, but failed to affect lipooxygenase-mediated metabolism, as assessed by the lack of increase in leukotriene C4 production; addition of TGFα- (T-CM) or EGF-conditioned medium to cultures of LHRH-producing GT1-1 cells stimulated LHRH release. In contrast, ... |
| Databáze: | OpenAIRE |
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