Arterial stiffness is associated with elastin fragmentation and medial collagen content in the human aorta

Autor: Azra Mahmud, Vincent Young, B. Silke, M. Tolan, E. McGovern, Pierce V. Kavanagh, John Feely, M McNulty, J P Spiers
Rok vydání: 2020
Předmět:
Zdroj: European Heart Journal. 41
ISSN: 1522-9645
0195-668X
DOI: 10.1093/ehjci/ehaa946.2706
Popis: Background Age-related changes in arterial stiffness are ascribed to collagen and elastin content in the aorta (Ao) which is modulated by the matrix metalloprotienases (MMPs). However, no study has directly compared arterial stiffness and arterial structure in man. Methods Aortic and internal mammary artery (IMA) tissue were obtained from 10 patients (62±1 years, 2 female) undergoing coronary artery bypass grafting (CABG). Aortic pulse wave velocity (PWV) was measured prior to CABG. Collagen content was assessed in tissue sections using Sirius Red staining and elastin by ACCUSTAIN. Elastin fragmentation in the Ao media was graded; increasing in severity from 1 to 4. MMP-2 and MMP-9 activity was quantified in the Ao using gelatine zymography. Results are expressed as mean±SEM, p Results The collagen concentration was 50% (intima), 42% (media)and 76% (adventitia) in the Ao but was lower in the IMA. PWV was significantly associated with Ao medial (r=0.79, p=0.03) but not intimal or adventitial collagen concentrations. Aortic intimal thickness was related significantly with age (r=0.70, p Conclusions Our proof-of-concept study is the first one to show that elastin fragmentation in the aorta may underlie arterial stiffening in man. In contrast, the internal mammary artery does not age, unlike the aorta, which makes it the reassuring choice for coronary artery bypass grafting. Funding Acknowledgement Type of funding source: Public Institution(s). Main funding source(s): Health Research Board, Ireland
Databáze: OpenAIRE
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