Prevalence and molecular distribution of Legionella pneumophila in cold water taps across Alborz province, Iran
| Autor: | Somayeh Yaslianifard, Maryam Arfaatabar, Mohammad Reza Pourmand, Mohammad Mohammadzadeh, Sahar Yaslianifard, Mehdi Norouzi, Mohammad Noori Sepehr |
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| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
030219 obstetrics & reproductive medicine biology Epidemiology medicine.drug_class Pseudomonas aeruginosa Antibiotics Public Health Environmental and Occupational Health Drug resistance Acinetobacter biology.organism_classification medicine.disease_cause medicine.disease Legionella pneumophila law.invention Microbiology 03 medical and health sciences 0302 clinical medicine Infectious Diseases Antibiotic resistance law medicine Legionnaires' disease 030212 general & internal medicine Polymerase chain reaction |
| Zdroj: | Clinical Epidemiology and Global Health. 7:525-529 |
| ISSN: | 2213-3984 |
| Popis: | Background The aim of this study is to explore the distribution of Legionella pneumophila (serogroups 1–6) in cold water taps across Alborz province, Karaj, Iran. In addition, some other non-fastidious gram-negative bacteria not belonging to the Enterobacteriaceae family (GNB-NE) were followed. Subject A total of 162 water samples were collected from 6 countries and tested by culture and genus-specific polymerase chain reaction (PCR). The L. pneumophila isolates were subsequently analyzed by monoclonal antibodies (MAbs) for serogroup identification, molecularly by PCR, and sequencing for genetic diversity. The patterns of resistance to antibiotics were determined by disk diffusion. Results Fifteen (9.25%) samples were detected as positive for L. pneumophila by PCR and 12 (7.4%) samples yielded growth in culture. All isolates of L. pneumophila were belonged to serogroup 1. Fifteen GNB-NE species were isolated from the samples, including nine isolates of Pseudomonas aeruginosa and six isolates of Acinetobacter spp. Antibiotic resistance was detected in 3 isolates of P. aeruginosa. Conclusions Despite the prevalence of L. pneumophila, few clinical cases have been reported, which may be due to underdiagnosis of the disease caused by it. Therefore, the prompt attention should be paid to do clinical diagnosis of legionellosis by the effective diagnostic tests from patients with compatible clinical symptoms. |
| Databáze: | OpenAIRE |
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