Cellulase production to obtain biogas from passion fruit (Passiflora edulis) peel waste hydrolysate
| Autor: | Liliana Andréa dos Santos, Anderson Felipe Viana da Silva, M. A. da Motta Sobrinho, José Fernando Thomé Jucá, Rebeca Beltrão Valença, Tatiana Souza Porto, Guilherme Gomes, André Felipe Melo Sales Santos |
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| Rok vydání: | 2019 |
| Předmět: |
biology
Chemistry Process Chemistry and Technology 02 engineering and technology Biodegradable waste Cellulase 010501 environmental sciences 021001 nanoscience & nanotechnology Pulp and paper industry biology.organism_classification 01 natural sciences Pollution Hydrolysate Passiflora Hydrolysis Biogas Enzymatic hydrolysis biology.protein Chemical Engineering (miscellaneous) Fermentation 0210 nano-technology Waste Management and Disposal 0105 earth and related environmental sciences |
| Zdroj: | Journal of Environmental Chemical Engineering. 7:103510 |
| ISSN: | 2213-3437 |
| DOI: | 10.1016/j.jece.2019.103510 |
| Popis: | Enzyme usage for valorization of organic and agroindustrial wastes is a biotechnology field that had been increasing due to economic and environmental benefits that add to productive cycles. Enzymatic hydrolysis is one of the possible pre-treatments used with the aim of facilitating lignocellulosic waste biodigestion. This work evaluated cellulase production by Aspergillus japonicus URM5620 and its use as enzymatic pre-treatment on passion fruit peel waste (Passiflora edulis) in order to facilitate the anaerobic biodigestion for biogas generation purposes. Cellulases were produced by submerged fermentation, being maximized using a factorial 2² design, analysing substrate concentration (passion fruit peel) (1.0 to 3.0%) and glucose (0.5 to 1.0%). Enzymatic hydrolyzate obtained from submerged fermentation was biodigested in anaerobic reactor, in a laboratory scale. The highest activities of 1.2 U/mL total cellulase (FPase) and 1.7 U/mL endoglucanase (CMCase) were obtained at 3% of substrate and 1% of glucose. Biogas production from hydrolyzed waste provided methane with a concentration of above 64% by volume after 15 days. Fermentation pre-treatment, using Aspergillus japonicus, was a step that potentially facilitates and accelerates lignocellulosic organic waste biodigestion. |
| Databáze: | OpenAIRE |
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