Genome modifications in protoplast-derived tobacco plants: Contents of repetitive DNA sequences

Autor:	Milan Bezděk, Jiří Fajkus, J. Soška, J. Reich, B. Vyskot
Rok vydání:	1991
Předmět:	Genome instability Genetics Nicotiana tabacum Repetitive Sequences Plant Science Horticulture Biology Protoplast biology.organism_classification Genome chemistry.chemical_compound chemistry Repeated sequence Solanaceae DNA
Zdroj:	Biologia plantarum. 33
ISSN:	1573-8264 0006-3134
Popis:	Plasticity of the tobacco genome was studied by testing the DNAs of protoplast-derived regenerants with three different repetitive DNA sequences by the method of quantitative DNA/DNA hybridizations. A large population of 91 regenerants belonging to 35 different protoclones was analysed and a high degree of heterogeneity in the contents of the different DNA repeats was detected. The contents of middle repetitive sequences of two types were more stable or changed in the same direction, while the highly repetitive sequence varied independently and displayed a significant reduction in comparison with the two other sequences. Comparing the variation within the subpopulations of plants of the same clonal origin and the variation among the protoclones led to a conclusion that the pre-existing DNA variability in the starting plant material and/or thein vitro stress during the very early stages of protoclone regeneration played a decisive role in the formation of modified genomes in regenerants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bf36f0b892a0b65c19d6c441b5945cb1 https://doi.org/10.1007/bf02897717 Zobrazit plný text záznamu Full text from SpringerLink