Identification of an Antigenic Determinant on Anionic Peanut Peroxidase by Monoclonal Antibodies
| Autor: | Yijun Xu, R. B. van Huystee |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Performic acid biology Physiology medicine.drug_class Plant Science Oligosaccharide Monoclonal antibody Trypsin Molecular biology Epitope chemistry.chemical_compound chemistry Biochemistry biology.protein medicine Peroxidase Conformational epitope Cysteine medicine.drug |
| Zdroj: | Journal of Experimental Botany. 42:935-945 |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/42.7.935 |
| Popis: | Two monoclonal antibodies (46-12-C12 and 23-6-C12) raised against anionic peanut peroxidase were found to have independent epitope sites. These topographic sites were found to be located within a tryptic glycopeptide (Atgp) from the anionic isozyme by both indirect and non-competive ELISA and Western blotting. The Atgp has a Mr equal to 11 000 of which 70% is carbohydrate and the peptide is probably highly hydrophobic as determined by its high RF (0-83) value and the amino acid composition. McAb 23-6-C12 recognized a contiguous epitope which encompassed also the sole N-linked oligosaccharide on the anionic isozyme. That the monoclonal antibody also recognized the oligosaccharide on the «-amylase, /3-glucosidase, acid phosphatase, and horse-radish peroxidase may be related to similarities in sugars. Sugar removal from the Atgp or from the cross-reactive peptide of enzymes caused loss of antibody affinity. The monoclonal antibody 46-12-C12 recognized specifically a conformational epitope near the region of the cysteine, tryptophane and methionine residue on Atgp. Digestion of the anionic isozyme by trypsin resulted in a 40-fold loss of affinity with this monoclonal antibody. Moreover, treatment of the Atgp with performic acid or trifluoromethane sulphonic acid caused a loss of affinity between the treated Atgp and this monoclonal antibody. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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