MyoD, myogenin and proliferating cell nuclear antigen expression in growing Nile tilapia (Oreochromis niloticusL.)

Autor:	Danilo Henrique Aguiar, Carlos Roberto Padovani, Cláudio Luis Bock, Maeli Dal Pai-Silva
Rok vydání:	2008
Předmět:	medicine.medical_specialty biology Aquatic Science biology.organism_classification MyoD Proliferating cell nuclear antigen Muscle hypertrophy Andrology Nile tilapia Oreochromis Endocrinology Internal medicine Myogenic regulatory factors biology.protein medicine Juvenile Myogenin
Zdroj:	Aquaculture Research.
ISSN:	1365-2109 1355-557X
DOI:	10.1111/j.1365-2109.2008.02042.x
Popis:	In the present study, immunohistochemical and morphometric analysis was used to characterize variations in muscle growth performance during muscle fibre recruitment and hypertrophy. As in fisheries, fish were classified into four age stages: alevin 35 days (0.65±0.08 g); juvenile 60 days (13.67±1.35 g); adult 90 days (73.18±4.70 g) and adult 190 days (349.76±34.62 g). The number of nuclei expressing MyoD and myogenin was similar in alevin, juvenile and adult 90 days; however, in adult 190 days, the number of nuclei expressing myogenin was higher than the number expressing MyoD. The number of proliferating cell nuclear antigen-stained nuclei in each stage was higher than MyoD and myogenin staining with peaks in alevin and adult 90 days. These data suggest that growth per se stimulated cellular proliferation and nuclei accretion of Nile tilapia muscle fibres in alevin, juvenile and adult 90 days. Muscle fibre differentiation was more pronounced in adult 190 days.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bf2388e7c98101ca3a217d79141ab53f https://doi.org/10.1111/j.1365-2109.2008.02042.x Zobrazit plný text záznamu