Leucocytes and cardiopulmonary bypass: in vitro production of oxygen free radicals and trapping in the reperfused myocardium
| Autor: | J. Vaage, Dag Sørlie, Ole D. Mjøs, A.G. Semb, M. Lie |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Radical 030204 cardiovascular system & hematology law.invention 03 medical and health sciences Coronary circulation Coronary artery bypass surgery 0302 clinical medicine law Internal medicine Cardiopulmonary bypass Medicine Radiology Nuclear Medicine and imaging Coronary sinus Advanced and Specialized Nursing business.industry General Medicine In vitro medicine.anatomical_structure 030228 respiratory system Anesthesia Cardiology Arterial blood Cardiology and Cardiovascular Medicine business Safety Research Ex vivo |
| Zdroj: | Perfusion. 5:169-180 |
| ISSN: | 1477-111X 0267-6591 |
| DOI: | 10.1177/026765919000500303 |
| Popis: | The production of oxygen free radicals (OFR) by leucocytes was evaluated ex vivo by chemiluminescence (CL) before, during and after routine coronary artery bypass surgery (group A, n=11). The possibility of leucocyte trapping in the coronary circulation during the early reperfusion period was also investigated (group B, n=9). In group A, arterial blood samples were taken immediately before the start of surgery during anaesthesia, five minutes before and five and 30 minutes after the start of cardiopulmonary bypass (CPB), five minutes before and five and 30 minutes after the start of reperfusion of the heart, and then four and 24 hours after the end of CPB. In group B, arterial and coronary sinus blood samples were simultaneously drawn five and 30 minutes after the release of the aortic crossclamp. All blood samples were corrected for haemodilution. In group A, both CL and the level of circulating leucocytes declined during CPB. The lowest value of CL was measured 30 minutes after the start of CPB (69± 2% of baseline values) (mean±SEM). The lowest level of leucocytes was found after 30 minutes of CPB: 2.6±0.4 (109/l) vs 4.2±0.5 before surgery. Twenty-four hours after CPB, CL was increased to 170±49% and a leucocytosis was present (12.2±1.1). In group B, after five minutes of reperfusion the number of circulating leucocytes in arterial blood was 3.8±0.9 x 109/l as compared to 2.2±0.5 x 109/l in the coronary sinus (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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