A water-soluble boronate masked benzoindocyanin fluorescent probe for the detection of endogenous mitochondrial peroxynitrite in live cells and zebrafish as inflammation models
| Autor: | Prasad M. Sonawane, Yunsu Kim, Tesla Yudhistira, Ju Hyeon Kim, Vikas K. Bhosale, Yeu-Chun Kim, David G. Churchill, Sachin S. Surwase, Arkaprava Roychaudhury, Cheol-Hee Kim, Hee-Sung Park, Mahesh B. Halle |
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| Rok vydání: | 2021 |
| Předmět: |
biology
Process Chemistry and Technology General Chemical Engineering Inflammation Endogeny 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Fluorescence 0104 chemical sciences chemistry.chemical_compound Water soluble chemistry Organelle medicine Biophysics Naked eye medicine.symptom 0210 nano-technology Zebrafish Peroxynitrite |
| Zdroj: | Dyes and Pigments. 191:109371 |
| ISSN: | 0143-7208 |
| DOI: | 10.1016/j.dyepig.2021.109371 |
| Popis: | Endogenous peroxynitrite a highly reactive oxidant plays significant roles in various physiopathological processes in living organisms. Therefore, sensing and monitoring of peroxynitrite with accurate detection specifically, is an immense challenge. To address this issue, we synthesized a water soluble, mitochondria-targeting “turn-on” fluorescent probe (BICBzBF) for the detection of mitochondrial peroxynitrite. BICBzBF (pale yellow) demonstrated high sensitivity and selectivity in fluorometric tracing of peroxynitrite over other ROS/RNS tested. This arylboronate-masked probe responds to peroxynitrite, resulting in the corresponding phenol derivative; a strong fluorescence accompanied a change to red rose as observed by the naked eye. The probe responds to peroxynitrite within |
| Databáze: | OpenAIRE |
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