Eukaryotic diversity in historical soil samples

Autor:	Willem M. de Vos, Georg W.M. van der Staay, Johannes H. P. Hackstein, Hauke Smidt, Seung Yeo Moon-van der Staay, Vesela A. Tzeneva
Rok vydání:	2006
Předmět:	Gel electrophoresis Ecology Microorganism Ribosomal RNA Biology biology.organism_classification Applied Microbiology and Biotechnology Microbiology 18S ribosomal RNA Botany Protozoa Soil microbiology Ribosomal DNA Temperature gradient gel electrophoresis
Zdroj:	FEMS Microbiology Ecology. 57:420-428
ISSN:	1574-6941 0168-6496
DOI:	10.1111/j.1574-6941.2006.00130.x
Popis:	The eukaryotic biodiversity in historical air-dried samples of Dutch agricultural soil has been assessed by random sequencing of an 18S rRNA gene library and by denaturing gradient gel electrophoresis. Representatives of nearly all taxa of eukaryotic soil microbes could be identified, demonstrating that it is possible to study eukaryotic microbiota in samples from soil archives that have been stored for more than 30 years at room temperature. In a pilot study, 41 sequences were retrieved that could be assigned to fungi and a variety of aerobic and anaerobic protists such as cercozoans, ciliates, xanthophytes (stramenopiles), heteroloboseans, and amoebozoans. A PCR-denaturing gradient gel electrophoresis analysis of samples collected between 1950 and 1975 revealed significant changes in the composition of the eukaryotic microbiota.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bec7a884ddb5a33c8a2a700ec4b83abc https://doi.org/10.1111/j.1574-6941.2006.00130.x Zobrazit plný text záznamu Plný text