Biological Characteristics of Aerosols Emitted From A Layer Operation In Southeastern U.S

Autor: Lingjuan Wang, Qianfeng Li, Otto D. Simmons, John J. Classen, Keith Esch, David B Beasley
Rok vydání: 2009
Předmět:
Zdroj: 2009 Reno, Nevada, June 21 - June 24, 2009.
DOI: 10.13031/2013.28035
Popis: This preliminary study was to investigate biological characteristics of aerosols emitted from a commercial layer farm (egg production farm). Bioaerosol samples were taken on this farm at five sampling locations covering emission source (inside a layer barn) and four ambient stations at four wind directions. All glass impingers (AGI) were used for the field sampling. The AGI fluid samples were plated in duplicate on Trypticase Soy Agar (TSA) for growth of bacteria and Sabouraud Dextrose Agar (SDA) for growth of fungi. The most prominent bacterial colony types were identified using a combination of methods that include recording characteristics of colony morphology; performing a Gram staining method and metabolic analyses using the Biolog system. Results from one group of bioaerosol samples at the five stations indicate that the sampling duration played an important role in accurately determining bacteria concentration in air samples; there were significant reductions in total bacteria concentrations in the samples collected from ambient stations compared to the sample collected in the layer house; the most prominent bacteria species differed among all five stations and three of the most prominent bacteria from samples taken at all five stations were gram-positive; fungal type differed from station to station.
Databáze: OpenAIRE