Effect of temperature and inoculation strategy on Cu recovery and microbial activity in column bioleaching
| Autor: | Helen R. Watling, Peter D. Franzmann, J.J. Johnson, Peter D. Nichols, John A. E. Gibson, D.W. Shiers, Jason J. Plumb |
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| Rok vydání: | 2016 |
| Předmět: |
biology
Chemistry Inoculation Microorganism Metals and Alloys Population development 02 engineering and technology 010501 environmental sciences biology.organism_classification 01 natural sciences Acclimatization Industrial and Manufacturing Engineering 020501 mining & metallurgy Lag time 0205 materials engineering Bioleaching Materials Chemistry Food science Temperature gradient gel electrophoresis Bacteria 0105 earth and related environmental sciences |
| Zdroj: | Hydrometallurgy. 164:189-201 |
| ISSN: | 0304-386X |
| DOI: | 10.1016/j.hydromet.2016.05.017 |
| Popis: | The effect of increasing temperature from 30 to 60 °C on iron (II)-oxidation activity and microbial population development in columns was investigated. With respect to ‘mode of inoculation’, pre-inoculation of the ore with a mixed culture resulted in a shorter lag time during which the microorganisms established themselves on the ore. Inoculation with the bacteria was successful using current techniques but inoculation with hyper-thermophiles appears to require special enrichment and acclimatisation strategies to maximise success. Staged inoculation of bacteria at their preferred temperatures did not confer additional advantage. Inoculation during acid agglomeration was impacted by exposure to initially high acidity. With respect to population development, the combined tracking methods (signature lipids and denaturing gradient gel electrophoresis (DGGE)) provided valuable insights into the thermal tolerance of some microbial strains in a column environment and also detected new strains. |
| Databáze: | OpenAIRE |
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