VIDAS® Enzyme-Linked Immunofluorescent Assay for Detection of Listeria in Foods: Collaborative Study

Autor:	V Atrache, Michael S Curiale, Ann M. Schultz, Vidhya Gangar, A D'Onorio, Ronald L. Johnson
Rok vydání:	2000
Předmět:	Pharmacology biology False Negative Reactions Elisa assay biology.organism_classification Analytical Chemistry Microbiology Listeria species Immunoenzyme techniques Listeria Environmental Chemistry Food microbiology Agronomy and Crop Science Food Science
Zdroj:	Journal of AOAC INTERNATIONAL. 83:903-918
ISSN:	1944-7922 1060-3271
DOI:	10.1093/jaoac/83.4.903
Popis:	The VIDAS LIS method and the traditional culture methods for detection of Listeria species in food were evaluated in a multilaboratory comparative study. The 6 foods tested were either naturally contaminated or inoculated with 3 different concentrations of Listeria. Results for each food and each contamination level with the VIDAS LIS method were as good as or better than those obtained with the traditional culture method. Of 1558 samples tested, 935 were positive: 839 by the VIDAS method and 809 by standard culture methods. Overall false negative rates were 10.3 and 13.5% for the VIDAS LIS and culture methods, respectively. The false positive rate for the VIDAS LIS assay was 1.4% based on 9 VIDAS LIS positive assays that did not confirm positive by isolation of Listeria. The agreement between the VIDAS LIS and culture methods for all samples tested was 86%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bead3b07036411c2c38f83d4a98262d5 https://doi.org/10.1093/jaoac/83.4.903 Zobrazit plný text záznamu