Cloning and Functional Expression of cDNA Encoding Pheromone ?9 Acyl-CoA Desaturase of the Tobacco Cutworm, Spodoptera litura (Lepidoptera: Noctuidae)

Autor: Park Hee Yun, Jeong Seong Eun
Rok vydání: 2005
Předmět:
Zdroj: Entomological Research. 35:253-263
ISSN: 1748-5967
1738-2297
DOI: 10.1111/j.1748-5967.2005.tb00167.x
Popis: A cDNA encoding pheromone Δ9 acyl-CoA desaturase, Slit KPSE was isolated from sex pheromone gland of the tobacco cutworm, Spodoptera litura which uses a diene unsaturated fatty acid (UFA) derivative, Z9E11-14 : 2 as a major pheromone component. The fulllength open reading frame coding region of Slit KPSE was inserted in a yeast shuttle vector, YEpOLEX, and two kinds of yeast (Saccharomyces cerevisiae) mutant strains were transformed with the recombinant vector. In the desaturase-deficient ole1 strain, Slit KPSE expressed a complementary enzyme producing two kinds of diene UFAs, more 9–16 : 1 and less 9–18 : 1 at a ratio of 1 : 0.74 exhibiting a typical functional characteristics as one of the pheromone Δ9 acyl-CoA desaturase lineage group, KPSE, but no Δ9 14C monoene was detectable because of too small amount of 14C saturated fatty acid precursor to be reliably used by Slit KPSE in the transformed cells. However, the another transformed yeast strain elo1 which is deficient of elongase 1, an enzyme converting 14C to 16C hydrocarbon substrate, was supplemented with some myristic acid (14 : 0) in the medium, and produced a significant amount of 9–14 : 1 in due to a much enhanced level of the 14C substrate suggesting that Slit KPSE may be responsible for making the Δ9 double bond on the diene pheromone component.
Databáze: OpenAIRE
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