Identification of Some Anion Transporter Genes in the Halophyte Suaeda altissima (L.) Pall. and Their Expression under Nitrate Deficiency and Salinity

Autor: I. V. Karpichev, L. A. Khalilova, A.V. Shuvalov, O.I. Nedelyaeva, Andrey A. Yurchenko, L.G. Popova, Yu. V. Balnokin, N. A. Myasoedov
Rok vydání: 2021
Předmět:
Zdroj: Russian Journal of Plant Physiology. 68:873-882
ISSN: 1608-3407
1021-4437
DOI: 10.1134/s1021443721050174
Popis: In the study, the partial sequences of several nitrate transporter/channel genes of the NPF, NRT2, NAR2 and SLAC/SLAH families from the halophyte Suaeda altissima (L.) Pall. were identified, and their relative transcript abundance in S. altissima plants grown in nutrient media with different concentrations of $${\text{NO}}_{3}^{ - }$$ and Cl– was examined by qRT-PCR. Identification of the partial S. altissima nitrate transporter sequences were carried out assuming similarity of putative S. altissima genes with homologous genes from the halophytes S. fruticosa and S. glauca, which are closely related to S. altissima. Nucleotide sequences of S. fruticosa and S. glauca homologous genes were obtained by in silico analysis of the de novo assembled transcriptomes of these halophytes. The short-read RNA arrays for the transcriptome assemblies were taken from the BioProject database ( https://www.ncbi.nlm.nih.gov/bioproject/ ), NCBI, Acc. no. #PRJNA279962 and #PRJNA295637. Based on the identified contigs in the assembled S. fruticosa and S. glauca transcriptomes that were characterised as putative nitrate transporters or channels, primer sets were designed for the amplification of sequences encoding S. altissima nitrate transporters: SaNPF6.3 (MK 580125.1), SaNRT2.1 (MK 580128.1), SaNRT2.5 (MK 580129.1), SaNAR2.1 (MK 580130.1), SaSLAH1.1 (MK 580131.1) and SaSLAH1.2 (MK 580132.1) (GenBank Acc. no. are given, https://www.ncbi.nlm.nih.gov/genbank/ ). It was shown that the expression of S. altissima genes under consideration is organ-specific and changed in response to changes in NO3ˉ and NaCl concentrations in the growth medium.
Databáze: OpenAIRE
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