RNA accumulation and genes expressed in spores of the arbuscular mycorrhizal fungus,Gigaspora rosea
| Autor: | Gilbert Meyer-Gauen, Philipp Franken, Vivienne Gianinazzi-Pearson, Laurence Lapopin |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hydrogenase Physiology Fungus 010603 evolutionary biology 01 natural sciences law.invention Microbiology 03 medical and health sciences law Complementary DNA Genetics Molecular Biology Gene Ecology Evolution Behavior and Systematics Polymerase chain reaction Messenger RNA biology fungi RNA Cell Biology General Medicine 030108 mycology & parasitology biology.organism_classification Molecular biology Spore |
| Zdroj: | Mycologia. 89:293-297 |
| ISSN: | 1557-2536 0027-5514 |
| DOI: | 10.1080/00275514.1997.12026784 |
| Popis: | RNA could be extracted from ungerminat- ed spores of the arbuscular mycorrhizal fungus Gigas- pora rosea with a maximum of yield 7 days after isola- tion from soil. cDNA was synthesized and used for li- brary construction or as template for the amplification by polymerase chain reaction of fragments corre- sponding to several genes. Analysis of cDNA clones corresponding to fragments of ribosomal RNA genes indicated that the extracted RNA as starting material was of arbuscular mycorrhizal fungal origin. Polymer- ase chain reaction showed the presence of mRNA from genes encoding glyceraldehyde-3-phosphate de- hydrogenase, fi-tubulin and two types of ATPases; mRNA accumulation of the glyceraldehyde-3-phos |
| Databáze: | OpenAIRE |
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