Resazurin assay for rapid drug susceptibility testing of Mycobacterium tuberculosis
| Autor: | Mangala Ghatole, Virendra Kashetty, Apeksh Ghule |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Drug
Tuberculosis biology business.industry media_common.quotation_subject 010401 analytical chemistry Isoniazid Rifamycin Resazurin Drug susceptibility biology.organism_classification medicine.disease 01 natural sciences 0104 chemical sciences Microbiology Mycobacterium tuberculosis chemistry.chemical_compound chemistry Pulmonary tuberculosis medicine business media_common medicine.drug |
| Zdroj: | Indian Journal of Microbiology Research. 5:138-142 |
| ISSN: | 2394-5478 2394-546X |
| DOI: | 10.18231/2394-5478.2018.0028 |
| Popis: | Introduction: Tuberculosis, a disease of antiquity continues to cause high morbidity and mortality. In Sustainable Development Goals and End Tb Strategy proposed by WHO includes Drug Susceptibility Testing as major component. Conventional methods are time consuming and molecular methods are rapid but expensive and demanding. Resazurin assay is a rapid colorimetric redox indicator method of Drug Susceptibility Testing. Materials and Methods: 67 strains of M. tuberculosis isolated from pulmonary tuberculosis patients were tested using indirect absolute concentration method by resazurin assay and compared with drug incorporated Lowenstein – Jensen media for Isoniazid and Rifamycin. Results: There was 91.5 % and 91.7% concordance between both methods for INH and Rifamycin respectively. Resazurin assay for INH was 100% sensitive and 61.54% specific. Sensitivity and specificity for RMP was 98.04% and 62.5% respectively. The duration required for INH was 7.0 ± 2.07 and for RMP 6.58 ± 1.58 days. Conclusions: Resazurin assay is sensitive, rapid, cheap, technically simple drug susceptibility method. Keywords: M. tuberculosis, resazurin assay, drug susceptibility. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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