FURTHER STUDY OF AN ACTH-RELEASING PROTEIN FROM HYPOPHYSEAL PORTAL VESSEL PLASMA1
| Autor: | Herbert W. Rumsfeld, John C. Porter |
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| Rok vydání: | 1959 |
| Předmět: | |
| Zdroj: | Endocrinology. 64:948-954 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-64-6-948 |
| Popis: | Fraction III0 obtained from hypophyseal portal vessel plasma by the Cohn fractionation procedure possessed all of the ACTH-releasing activity of portal plasma. This fraction consisted of three major protein components with isoelectric points at pH 4.4, pH 5.1 and pH 6.6. Separation of the components of this fraction by starch column electrophoresis yielded an active peak with a mobility in veronal buffer (pH 8.6; µ = 0.l) of −1.91±.41 cm.2 volt−1 sec.−1 ×10−5. The activity associated with the proteins of fraction III0 was destroyed by incubation with trypsin and pepsin and by refluxing with 0.1 N NaOH. It was not destroyed by two hours of refluxing with 0.1 N HC1. Preliminary results indicated that the active substance could be dialyzcd awajr from the proteins in distilled water if the lyophilized portal plasma was dissolved in a Cohn Buffer at pH 6.9. This would suggest that this plasma protein serves in a transport role for an ACTH-releasing substance in portal vessel blood. |
| Databáze: | OpenAIRE |
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