Monitoring male reproductive success in a Japanese black pine clonal seed orchard with RAPD markers
| Autor: | Y Ide, F Miyahara, S Goto |
|---|---|
| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Canadian Journal of Forest Research. 32:983-988 |
| ISSN: | 1208-6037 0045-5067 |
| DOI: | 10.1139/x02-022 |
| Popis: | Overall male reproductive success was investigated in a Japanese black pine (Pinus thunbergii Parl.) clonal seed orchard consisting of 16 nematode-resistant clones, using random amplified polymorphic DNA (RAPD) markers. We genotyped all 16 of the orchard clones at selected RAPD loci by analyzing diploid needles and 10 haploid megagametophytes from each of them. In addition, the male parent of open-pollinated seedlings was identified by comparing their phenotypes with the genotypes of the orchard clones. In this way the male parents of 559 seedlings of 648 seedlings of progenies from 14 parental (orchard) clones were determined. The level of external pollen contamination in the seed orchard was estimated to be 2.2% for the total population. The effective mean selfing rate observed was 1.5%. Male reproductive success among clones varied widely, from 0.8 to 24.4%, for the total population. Our data suggest that the significant deviation in male reproductive success of individual clones from panmictic ratios may be a problem for the genetic quality in seed orchard crops. To improve the parental balance of seed orchard crops, the use of artificial management techniques such as gibberellin A4/7 treatment, supplemental mass pollination, and rearrangement of clonal ramets is recommended in seed orchards. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|