Neither absence nor excess of ? O initiator-digesting ClpXP protease affects ? plasmid or phage replication in Escherichia coli

Autor:	Grzegorz Węgrzyn, Karol Taylor, Agnieszka Szalewska
Rok vydání:	1994
Předmět:	Protease biology medicine.medical_treatment DNA replication Lambda phage medicine.disease_cause biology.organism_classification Microbiology Molecular biology DnaA Bacteriophage chemistry.chemical_compound Plasmid chemistry medicine Molecular Biology Escherichia coli DNA
Zdroj:	Molecular Microbiology. 13:469-474
ISSN:	1365-2958 0950-382X
DOI:	10.1111/j.1365-2958.1994.tb00441.x
Popis:	Owing to rapid proteolysis of the coliphage lambda-coded initiator protein, lambda O, this protein is considered to carry a rate-limiting step in lambda DNA replication. The discovery of ClpXP protease responsible for lambda O protein turnover allowed an opportunity to verify this hypothesis. However, neither absence nor excess of this protease significantly affected the transformation efficiency and copy number of lambda plasmid, or the kinetics of the lambda phage growth. These results are also incompatible with the hypothesis that the stabilization of lambda O plays a role in the switch from early (circle-to-circle) to late (rolling-circle) lambda phage DNA replication. Transcriptional activation of ori lambda, probably assisted by the Escherichia coli DnaA function, remains as the possible rate-limiting step in lambda DNA replication.
Databáze:	OpenAIRE
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