Endocytosis frustration potentiates compression-induced receptor signalling
| Autor: | Samuel Tettarasar, Francesco Baschieri, Nadia Elkhatib, Dahiana Le Devedec, Guillaume Montagnac |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
MAPK/ERK pathway
0303 health sciences biology Endocytic cycle Cell Cell Biology Endocytosis Clathrin Cell biology 03 medical and health sciences Paracrine signalling 0302 clinical medicine medicine.anatomical_structure medicine biology.protein Epidermal growth factor receptor Receptor 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Journal of Cell Science. |
| ISSN: | 1477-9137 0021-9533 |
| Popis: | Cells experience mechanical stresses in different physiological and pathological settings. Clathrin-coated structures (CCSs) are sensitive to such perturbations in a way that often results in a mechanical impairment of endocytic budding. Compressive stress is a mechanical perturbation that leads to increased membrane tension and promotes proliferative signals. Here, we report that compression leads to CCSs frustration and that CCSs are required to potentiate receptor-mediated signaling in these conditions. We show that cell compression stalled CCSs dynamics and slowed down the dynamic exchange of CCSs building blocks. As previously reported, compression-induced paracrine activation of the epidermal growth factor receptor (EGFR) was the primary cause of ERK activation in these conditions. We observed that the EGFR was efficiently recruited at CCSs upon compression and that CCSs were required for full ERK activation. In addition, we demonstrated that compression-induced frustrated CCSs could also increase ligand-dependent signaling of other receptors. We thus propose that CCS frustration resulting from mechanical perturbations can potentiate signaling through different receptors with potential important consequences on cell adaptation to its environment. |
| Databáze: | OpenAIRE |
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