Evaluation of in vitro mucilage and lepidine biosynthesis in different genotypes of Lepidium sativum Linn originated from Iran
| Autor: | Pooran Golkar, F. Hadian, M. Koohi-Dehkordi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Plant Science Biology 01 natural sciences In vitro 0104 chemical sciences Lepidine Lepidium sativum 010404 medicinal & biomolecular chemistry Tissue culture chemistry.chemical_compound Horticulture Biosynthesis chemistry Mucilage Callus Medicinal plants 010606 plant biology & botany |
| Zdroj: | South African Journal of Botany. 127:91-95 |
| ISSN: | 0254-6299 |
| DOI: | 10.1016/j.sajb.2019.08.028 |
| Popis: | Garden cress (Lepidium sativum L.) is considered as a medicinal plant with various applications in pharmaceutical and food industries. Mucilage and lepidine as the most secondary metabolites in garden cress were optimized by callus culture. Firstly, the callus was induced under the treatment of 2, 4-D (1 mg l−1) + BAP (2 mg l−1). Then, in vitro synthesis of mucilage and lepidine from the callus and seeds of 10 different garden cress genotypes were evaluated under two different of light conditions (light and darkness). The in vitro synthesis of mucilage and lipidine from callus samples showed higher efficiency in most Iranian genotypes by about two-fold increase compared with one in the seed and leaf tissues respectively. L.s(4) and L.s(9) genotypes with 246.7 and 73.8 mg g−1 Dw had the highest and lowest contents of mucilage in callus tissue. The content of lepidine in L.s(6) (12.16 g g−1Dw) and L.s(5) (1.05 g g−1Dw) genotypes showed the highest and lowest content compared to those of callus. The findings of this research can be applied to high-throughput production of mucilage and lepidine using in vitro callus culture in Lepidium sativum. |
| Databáze: | OpenAIRE |
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