Molecular breeding of a sake yeast with a mutated ARO4 gene which causes both resistance to o-fluoro-dl-phenylalanine and increased production of β-phenethyl alcohol

Autor:	Kozo Ouchi, Seigo Takasawa, Kazuro Fukuda, Makoto Watanabe, Kozo Asano
Rok vydání:	1992
Předmět:	chemistry.chemical_classification biology Saccharomyces cerevisiae Phenylalanine biology.organism_classification Applied Microbiology and Biotechnology Yeast Transformation (genetics) chemistry.chemical_compound Enzyme Biochemistry chemistry Phenethyl alcohol Fermentation Tyrosine Biotechnology
Zdroj:	Journal of Fermentation and Bioengineering. 73:366-369
ISSN:	0922-338X
DOI:	10.1016/0922-338x(92)90280-8
Popis:	A sake yeast, Saccharomyces cerevisiae, was transformed with a YCp vector containing a mutated ARO4 gene (ARO4-OFP) derived from S. cerevisiae which encodes the 3-deoxy- d -arabino-heptulosonate-7-phosphate (DAHP) synthase released from feedback inhibition by tyrosine. The transformant showed feedback-insensitive DAHP synthase activity and simultaneously acquired both o-fluoro- dl -phenylalanine (OFP)-resistance and increased production of β-phenethyl alcohol and tyrosine. The greater production of the alcohol and its acetate, rose-like flavor components, improved the aroma and flavor of sake prepared experimentally with the transformant. The ARO4-OFP gene was also demonstrated to be available as a dominant selection marker in yeast vectors for the transformation of industrial strains of S. cerevisiae.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::be46240cc29627b031b53a818172de3a https://doi.org/10.1016/0922-338x(92)90280-8 Zobrazit plný text záznamu