The ZF87/MAZ transcription factor functions as a growth suppressor in fibroblasts
| Autor: | Matthew C Stubbs, InSung Min, Marc W Izzo, Ravikumar Rallapalli, Assia Derfoul, David J Hall |
|---|---|
| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | Biochemistry and Cell Biology. 78:477-485 |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/o00-053 |
| Popis: | ZF87/MAZ is a zinc finger transcription factor that activates expression of tissue-specific genes and represses expression of the c-myc proto-oncogene. Infection of NIH3T3 fibroblasts with a retrovirus expressing ZF87/MAZ leads to a significant reduction in G418-resistant colonies, compared to cells infected with a retroviral control. Further, only a small fraction of the G418-resistant colonies express ZF87/MAZ. When the ZF87/MAZ-expressing colonies are expanded, they demonstrate a slow growth phenotype, a delayed transit through G1 phase and a decrease in endogenous c-myc gene expression and cyclin A and cyclin E protein levels. Consistent with a partial G1 arrest, the ZF87/MAZ-expressing cells show a reduced sensitivity to the S phase specific chemotherapeutic agent camptothecin. These data indicate that ZF87/MAZ is a growth suppressor protein in nontransformed cells, in part, by affecting the levels of key cell cycle regulatory proteins.Key words: cell cycle, ZF87/MAZ, cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|