DNA-protein interaction in HeLa cells in different phases of the mitotic cycle and in resting cells

Autor:	V. S. Shapot, A. V. Likhtenshtein, N. I. S'yakste, G. S. Kalendo
Rok vydání:	1982
Předmět:	biology Chemistry Cell General Medicine Cell cycle biology.organism_classification General Biochemistry Genetics and Molecular Biology Dissociation (chemistry) Chromatin HeLa chemistry.chemical_compound medicine.anatomical_structure medicine Urea Biophysics Protein–DNA interaction DNA
Zdroj:	Bulletin of Experimental Biology and Medicine. 94:1662-1664
ISSN:	1573-8221 0007-4888
Popis:	DNA in proliferating cells is very firmly bound with proteins (the II form) and to elute it from celite a high concentration of LiCI and urea (4 and 8 M respectively) and heating to a temperature of about 90~ are required. When the cells pass into the resting state, transition of the cell chromatin to a different type of DNA--protein interaction is observed, for which 1.5 M LiCI and 3 M urea at a temperature of 2-4~ are sufficient to produce dissociation of the complex (the I form). Transition of chromatin from the II into the I form was found to be coupled with transition of widely different types of cells from a state of division to rest.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::be36e27e0a87f8abd33289d9c8c1e80b https://doi.org/10.1007/bf00838902 Zobrazit plný text záznamu Full text from SpringerLink