Protective Effects of Hydroxysafflor Yellow A against Oxidative Damage of β-Mercaptoethanol During Neural Differentiation of Mesenchymal Stem Cells
| Autor: | Li-ning Su, Ying-hui Liu, Hai-feng Yin, Hui-ping Wei, Xiaoqing Song |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pharmacology Mesenchymal stem cell Glutathione Molecular biology In vitro Blot 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Complementary and alternative medicine chemistry Biochemistry Apoptosis Pharmacology (medical) Neural differentiation Viability assay Neural development 030217 neurology & neurosurgery |
| Zdroj: | Chinese Herbal Medicines. 9:282-288 |
| ISSN: | 1674-6384 |
| DOI: | 10.1016/s1674-6384(17)60105-9 |
| Popis: | Objective To study the protective effects of hydroxysafflor yellow A (HSYA) against the oxidative damage caused by β-mercaptoethanol (BME) during neural differentiation of mesenchymal stem cells (MSCs) in vitro. Methods When the confluence reached 50%-60%, 4 th passage MSCs were divided into three groups to culture. G1: normal group which was cultured using basic medium (DMEM containing 10% FBS all the time); G2: unprotected group which was continuously cultured using basic medium for 24 h, and then cultured using pre-induction medium (DMEM containing 10% FBS and 1 mmol/L BME); G3: protected group which was firstly cultured using protective medium (DMEM containing 10% FBS and 160 mg/L HSYA) for 24 h, and then cultured using pre-induction medium for 24 h. After these treatments as above, cell viability, relative levels of SOD/GSH and apoptosis rate were respectively detected. The expression of Bcl and Bax was examined by Western blotting. After HSYA protection and BME pre-induction, neural induction was performed. The expression of NSE and MAP-2 was respectively analyzed on cellular and molecular levels. Results Compared with unprotected group, 160 mg/L HSYA could obviously improve cells viability, maintain high level of SOD and GSH in MSCs, reduce apoptosis rate and improve the ratio of Bcl/Bax. After protection with 160 mg/L HSYA, the survival time of neuron-like cells could be extended. Immunocytochemical staining showed that after 10 h of neural induction, the differentiated neuron-like cells in protected group were still in a good state, and the mRNA levels of NSE and MAP-2 were increased during the induction course checked. Conclusion HSYA could improve the resistance of cells to the oxidative damage caused by BME. |
| Databáze: | OpenAIRE |
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