Probing the dynamic fluorescence properties of single water-soluble quantum dots
| Autor: | Peter Yim, Matthew L. Clarke, Jeffrey R. Krogmeier, Jeeseong Hwang, Hyeonggon Kang |
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| Rok vydání: | 2008 |
| Předmět: |
Materials science
business.industry technology industry and agriculture Fluorescence intermittency equipment and supplies Fluorescence Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials law.invention Optics Nanocrystal Confocal microscopy law Quantum dot Fluorescence microscope Surface modification Electrical and Electronic Engineering Physical and Theoretical Chemistry business Spectroscopy |
| Zdroj: | Optics Communications. 281:1781-1788 |
| ISSN: | 0030-4018 |
| DOI: | 10.1016/j.optcom.2007.07.068 |
| Popis: | The influence of surface modifications, including carboxyl-, amine-, and mercaptoundecanoicacid-functionalized chemistries, on the dynamic fluorescence properties of single quantum dots (QDs) are probed using a combined confocal fluorescence microscopy and spectroscopy system. Water-soluble QDs with carboxyl- or amine-functionalized surfaces prepared through a linker arm show significant fluorescence intermittency and appear statistically identical. In contrast, water-soluble QDs functionalized via mercaptoundecanoicacid, an electron-donating thiol group, demonstrate suppressed fluorescence intermittency suggesting the photostability of water-soluble QDs is strongly dependant upon the method of surface functionalization. In addition, the long-term photostability of the mercaptoundecanoicacid-functionalized QD demonstrates an excitation-time-dependent blue-shift in the fluorescence emission spectra. Our measurement and analysis techniques promise a wealth of insight in elucidating the photophysics of bio-conjugated QDs and assessing and designing bio-conjugated QDs for biomedical applications requiring long-term photostabililty. |
| Databáze: | OpenAIRE |
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