Alpha 2,3- and alpha 2,6-sialylation of human skim milk glycoproteins during milk maturation
| Autor: | Jolanta Lis-Kuberka, Marta Berghausen-Mazur, Magdalena Orczyk-Pawiłowicz |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
food.ingredient Glycoconjugate Health Toxicology and Mutagenesis Biomedical Engineering Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound fluids and secretions food Immune system Antigen Artificial Intelligence Lactation Skimmed milk medicine General Pharmacology Toxicology and Pharmaceutics chemistry.chemical_classification 030102 biochemistry & molecular biology General Immunology and Microbiology General Neuroscience food and beverages General Medicine Sialic acid carbohydrates (lipids) 030104 developmental biology medicine.anatomical_structure chemistry Biochemistry cardiovascular system Jacalin General Agricultural and Biological Sciences Glycoprotein |
| Zdroj: | Journal of Applied Biomedicine. 15:196-203 |
| ISSN: | 1214-0287 1214-021X |
| DOI: | 10.1016/j.jab.2017.02.001 |
| Popis: | Human milk is a source of glycoconjugates, sialylated forms of which enrich the newborn immature immune system and are crucial for their proper development and well-being. Here, we analyzed the expression of α2,3-/α2,6-sialylated glycotopes on skim milk glycoproteins over lactation. Milk samples were analyzed by lectin-blotting using α2,3- and α2,6- sialic acid specific Maackia amurensis (MAA) and Sambucus nigra (SNA) lectins and sialyl- and asialyl-T antigen specific Artocarpus integrifolia (Jacalin) and Arachis hypogaea (PNA) lectins. The reactivities of MAA, SNA, Jacalin and PNA with milk glycoproteins showed that they are heavily decorated with α2,3-/α2,6-linked sialic acid and sialyl-T antigen and to a lesser degree with asialyl-T antigen. Despite individual differences of particular glycoproteins, a sharp and significant decline of α2,6-sialylated glycotopes and sialyl-T antigens and a weaker but significant decrease of α2,3-sialylated glycotopes and asialyl-T antigens on milk glycoproteins during milk maturation was observed. The expression of α2,3-/α2,6-sialylated glycotopes, sialyl- and asialyl-T antigens corresponds to milk maturation but differs in relation to the analyzed glycoprotein. Sialylated milk glycoproteins are considered as a part of innate immunity provided to neonates. Further investigations are needed to understand if they may be useful in milk banking to control the biochemical quality of milk. |
| Databáze: | OpenAIRE |
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