| Autor: |
Lin Qi, Rongxin Liang, Jingjing Duan, Songze Song, Yunjun Pan, Hui Liu, Mingan Zhu, Lian Li |
| Rok vydání: |
2022 |
| Popis: |
Background: Resveratrol has been reported to have synergistic effect with different antibiotics against various kinds of bacteria species. In this study, we investigated if resveratrol can increase the antimicrobial efficacy of polymyxin B against multidrug resistant (MDR) Pseudomonas aeruginosa.7 different MDR Pseudomonas aeruginosa isolates obtained from clinical specimen were tested with broth microdilution method to determine their sensitivity to polymyxin B and resveratrol respectively. Checkerboard analysis was performed to analyze the biological interaction between resveratrol and polymyxin B. Time-kill assays were carried out to monitor the bactericidal activity against Pseudomonas aeruginosa cells. And the biofilm-forming bacteria were selected for biofilm formation analysis.Results: Though resveratrol showed no intrinsic bactericidal activity against Pseudomonas aeruginosa (MIC >512μg/ml for all isolates), it could effectively increase the sensitivity of those isolates to polymyxin B, with the MIC of polymyxin B reduced by 4-8 fold with 64μg/ml resveratrol. The synergistic effect between resveratrol and polymyxin B on bactericidal activity was also observed with all tested isolates (FICI P 32μg/ml). The bactericidal activity of polymyxin B against those cells protected by established biofilm could still be promoted by resveratrol (P Conclusions: Resveratrol was able to improve the antimicrobial properties of polymyxin B from various aspects, it may be an ideal partner for the combination therapy of polymyxin B against MDR Pseudomonas aeruginosa. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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