Photothermal effects on protein adsorption dynamics of PEGylated gold nanorods
| Autor: | Vida Araban, Morteza Mahmoudi, Beatriz Pelaz, Aitor Alvarez, Pablo del Pino, Pablo Taboada, Ester Polo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
biology
Protein Corona Isothermal titration calorimetry 02 engineering and technology Polyethylene glycol Photothermal therapy 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences chemistry.chemical_compound chemistry PEG ratio biology.protein Biophysics General Materials Science Nanorod Bovine serum albumin 0210 nano-technology Protein adsorption |
| Zdroj: | Applied Materials Today. 15:599-604 |
| ISSN: | 2352-9407 |
| Popis: | The effect of thermoplasmonic heating on the dynamics of the protein corona around polyethylene glycol (PEG) coated gold nanorods (GNRs) is reported. GNRs were surface-modified with densely packed shells composed of thiolated PEGs bearing carboxyl and/or amine end-terminal groups, which led to negatively, positively or neutral charged GNRs. The three types of PEGylated GNRs, although with subtle differences among them, present weak interactions (binding dissociation constants in the μM range) with the model protein bovine serum albumin (BSA), as determined by different methods including gel electrophoresis, laser Doppler anemometry and isothermal titration calorimetry (ITC). In general, plasmonic photothermal heating significantly changed the composition of the protein corona around GRNs, particularly, on the positively charged ones. Moreover, a pre-formed BSA corona on the PEGylated GNRs further decreased their already reduced uptake by cells. However, photothermal treatment of BSA-coated GNRs only produced a significant decreased internalization in the case of non-PEGylated control samples. Overall, our results indicate that differently surface charged PEGylations on GNRs affects the dynamics of protein corona formation, which is particularly relevant after photothermal treatment. Such effects are nonetheless non-significant concerning cellular uptake. |
| Databáze: | OpenAIRE |
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