Structural and regulatory genes for coli surface associated antigen 4 (CS4) are encoded by separate plasmids in enterotoxigenicEscherichia colistrains of serotype 025.H42

Autor:	H. R. Smith, Geraldine A. Willshaw, Moyra M. McConnell, B. Rowe
Rok vydání:	1990
Předmět:	Serotype Structural gene Biology medicine.disease_cause Microbiology Molecular biology Plasmid Regulatory sequence Enterotoxigenic Escherichia coli Genetics medicine Heat-stable enterotoxin Molecular Biology Escherichia coli Regulator gene
Zdroj:	FEMS Microbiology Letters. 68:255-260
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1990.tb13947.x
Popis:	Two enterotoxigenic Escherichia coli strains of serotype 0.25.H42 that produced coli surface associated antigens CS4 and CS6 hybridized with a probe containing the cfaD sequence that regulates expression of colonization factor antigen CFA/I. Transformation of a cloned cfaD gene into some derivatives of the strains that were negative for CS4 and CS6 resulted in expression of CS4 but not CS6. By hybridization the sequence that regulated CS4 production in the wild type 025 strains was located on a plasmid that also encoded the CS6 antigen. The structural genes for the CS4 antigen were on a separate plasmid. The 025 strains carried a third plasmid encoding enterotoxin production which was therefore unlinked to regulation sequences or genes encoding CS antigens.
Databáze:	OpenAIRE
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