Paper-based immunoaffinity devices for accessible isolation and characterization of extracellular vesicles

Autor: Chao-Min Cheng, Chihchen Chen, Bo-Ren Lin, Shu-Ting Fan, Hsi-Kai Wang, Min-Yen Hsu
Rok vydání: 2014
Předmět:
Zdroj: Microfluidics and Nanofluidics. 16:849-856
ISSN: 1613-4990
1613-4982
DOI: 10.1007/s10404-014-1359-1
Popis: We present a paper-based immunoaffinity platform for separating subsets of extracellular vesicles (EVs). This method is inexpensive, robust, easy-to-use (easy-to-prepare), and compatible with downstream analyses, such as scanning electron microscopy (SEM), enzyme-linked immunosorbent assays (ELISA), and transcriptome analysis. EVs are small membranous vesicles shed from both healthy and diseased cells and contain nucleic acid and protein cargo. EVs have been increasingly recognized as a means of cell–cell communication and hold a great potential for clinical applications. However, current protocols for isolation of EVs are often lengthy, cumbersome and require expensive equipment. Here, we have isolated EVs from small volumes of both human serum and aqueous humor samples using paper-based immunoaffinity devices. Captured EVs were analyzed morphologically using SEM and appeared statistically different in size (p value
Databáze: OpenAIRE
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