Isolation and molecular detection of duck plague virus for the development of vaccine seed
| Autor: | Sabiha Sultana Soma, KHM Nazmul Hussain Nazir, Md Tanvir Rahman, Md Mizanur Rahman, Mosammat Shamim Ara, Rebeka Sultana, Md Rifat Haydar, Mahbubul Pratik Siddique, Md Bahanur Rahman |
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| Rok vydání: | 2018 |
| Zdroj: | Asian-Australasian Journal of Bioscience and Biotechnology. 3:78-85 |
| ISSN: | 2414-6293 2414-1283 |
| DOI: | 10.3329/aajbb.v3i1.64758 |
| Popis: | The present study was carried out for the isolation and molecular detection of duck plague virus (DPV) for the development of inactivated vaccine seed from the local outbreaks. A total of 12 suspected dead duck samples were collected from commercial farms and local market at Sunamganj, Netrokona and Mymensingh districts. Then, the samples were processed and prepared inocula were inoculated into 9-12 days old duck embryonated eggs. In duck embryonated eggs, several passages (3-4) were performed before infection into DEF cell culture. Presence of viral DNA was confirmed by PCR using the primer for DNA polymerase gene. After PCR confirmation, virus cultured in DEF cell was used for the preparation of formalin (0.12%) inactivated and oil based adjuvanted vaccine and was experimentally injected to 18 ducklings and 5 were kept as control. TCID50 of the selected virus for vaccine preparation was 108.70/ml. The mean passive haemagglutination assay (PHA) titre of sera of samples at 0 days, 7 days and 14 days post vaccination were 4.0±0, 14.22±1.78 and 44.44±4.4, respectively, which indicated significant (p |
| Databáze: | OpenAIRE |
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